Reverse phenotyping
We stimulated T cells from COVID-19 patients with spike protein ex vivo and computationally integrated snapshots before and after stimulation using T cell clonotypes derived from single-cell sequencing of the VDJ region.
Fischer, D.S., Ansari, M., Wagner, K.I. et al. Single-cell RNA sequencing reveals ex vivo signatures of SARS-CoV-2-reactive T cells through ‘reverse phenotyping’. Nat Commun 12, 4515 (2021).